Other Names Antigen NY-CO-13, Cellular tumor antigen p53, Phosphoprotein p53, TP53, Tumor suppressor p53
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Stathmin around the phosphorylation site of threonine 81 (A-P-TP-P-A).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity p53 (Phospho-Thr81) antibody detects endogenous levels of Stathmin only when phosphorylated at threonine 81.
ReferencesLloyd M. Alderson, Cancer Res., Mar 1995; 55: 999 - 1001.
WE Mercer, PNAS, Aug 1990; 87: 6166.
Timothy J. Underwood, AACR Meeting Abstracts, Apr 2006; 2006: 1167.
Jean-Christophe Bourdon, Genes & Dev., Sep 2005; 19: 2122 - 2137.