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Primary Antibodies

p130 Cas (Phospho-Tyr249) Antibody

E11-0696ACite ID: EN0017415DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
P56945
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names BCA1, BCAR1, Breast cancer anti-estrogen resistance 1 protein, CAS, CRK-associated substrate, CRKAS
All Sites Human: Tyr249; Mouse: Tyr253; Rat: Tyr347
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human p130 Cas around the phosphorylation site of tyrosine 249 (D-I-YP-D-V).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity p130 Cas (Phospho-Tyr249) antibody detects endogenous levels of p130 Cas only when phosphorylated at tyrosine 249.
ReferencesBrinkman A., J. Natl. Cancer Inst. 92:112-120(2000).
Ota T., Nat. Genet. 36:40-45(2004).
Amanchy R., J. Proteome Res. 4:1661-1671(2005).
Price225$
Western blot analysis of extracts from HepG2 cells treated with EGF (200ng/ml, 30mins) and A549 cells treated with PMA (125ng/ml, 30mins), using p130 Cas (Phospho- Tyr249) antibody.
Western blot analysis of extracts from HepG2 cells treated with EGF (200ng/ml, 30mins) and A549 cells treated with PMA (125ng/ml, 30mins), using p130 Cas (Phospho- Tyr249) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0696A-Conjugate. Price: $485 / Size: 100μl.

E11-0696A-FITC$485 / 100μlE11-0696A-Biotin$485 / 100μlE11-0696A-HRP$485 / 100μlE11-0696A-AP$485 / 100μlE11-0696A-AF350$485 / 100μlE11-0696A-AF405M$485 / 100μlE11-0696A-AF488$485 / 100μlE11-0696A-AF514$485 / 100μlE11-0696A-AF532$485 / 100μlE11-0696A-AF555$485 / 100μlE11-0696A-AF568$485 / 100μlE11-0696A-AF405S$485 / 100μlE11-0696A-AF405L$485 / 100μlE11-0696A-AF546$485 / 100μlE11-0696A-AF594$485 / 100μlE11-0696A-AF610$485 / 100μlE11-0696A-AF635$485 / 100μlE11-0696A-AF647$485 / 100μlE11-0696A-AF680$485 / 100μlE11-0696A-AF700$485 / 100μlE11-0696A-AF750$485 / 100μlE11-0696A-AF790$485 / 100μlE11-0696A-APC$485 / 100μlE11-0696A-PE$485 / 100μlE11-0696A-Cy3$485 / 100μlE11-0696A-Cy5$485 / 100μlE11-0696A-Cy5.5$485 / 100μlE11-0696A-Cy7$485 / 100μlE11-0696A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.