Other Names BCA1, BCAR1, Breast cancer anti-estrogen resistance 1 protein, CAS, CRK-associated substrate, CRKAS
All Sites Human: Tyr249; Mouse: Tyr253; Rat: Tyr347
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human p130 Cas around the phosphorylation site of tyrosine 249 (D-I-YP-D-V).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity p130 Cas (Phospho-Tyr249) antibody detects endogenous levels of p130 Cas only when phosphorylated at tyrosine 249.
ReferencesBrinkman A., J. Natl. Cancer Inst. 92:112-120(2000).
Ota T., Nat. Genet. 36:40-45(2004).
Amanchy R., J. Proteome Res. 4:1661-1671(2005).