Other Names BCA1, BCAR1, Breast cancer anti-estrogen resistance 1 protein, CAS, CRK-associated substrate, CRKAS
All Sites Human: Tyr165; Mouse: Tyr169; Rat: Tyr263
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human p130 Cas around the phosphorylation site of tyrosine 165 (D-L-YP-Q-V).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity p130 Cas (Phospho-Tyr165) antibody detects endogenous levels of p130 Cas only when phosphorylated at tyrosine 165.
ReferencesSara Cabodi, Cancer Res., May 2006; 66: 4672 - 4680.
Päivi T. Lakkakorpi, J. Biol. Chem., Feb 1999; 274: 4900.
Zhiyuan Zhang, J. Biol. Chem., Aug 1999; 274: 25093.
Erguang Li, J. Biol. Chem., May 2000; 275: 14729.