World supplier of antibodies, antigens and kits since 2007
Primary Antibodies
NPM (Phospho-Thr199) Antibody
$225/ 100μg
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Other Names B23.1, NPM isoform 1, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin, nucleophosmin
All Sites Human: Thr199; Mouse: Thr198; Rat: Thr198
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human NPM around the phosphorylation site of threonine 199 (R-D-TP-P-A).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity NPM (Phospho-Thr199) antibody detects endogenous levels of NPM only when phosphorylated at threonine 199.
ReferencesPhilipp B Staber, Blood, Aug 2007; 10.1182/blood-2007-02-071258.
Brunangelo Falini, Blood, Nov 1999; 94: 3509 - 3515.
Jean-François Honorat, Blood, May 2006; 107: 4130 - 4138.
Kotaro Funato, Blood (ASH Annual Meeting Abstracts), Nov 2006; 108: 291.
Brunangelo Falini, Blood, Nov 1999; 94: 3509 - 3515.
Jean-François Honorat, Blood, May 2006; 107: 4130 - 4138.
Kotaro Funato, Blood (ASH Annual Meeting Abstracts), Nov 2006; 108: 291.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using NPM (Phospho- Thr199) antibody.
Western blot analysis of extracts from Jurkat cells, using NPM (Phospho-Thr199) antibody.
Immunofluorescence analysis of HeLa cells, treated with EGF (200nM, 5mins), using NPM (Phospho-Thr199) antibody.
Western blot analysis using NPM (Phospho-Thr199) Antibody. Line 1: Blocking peptide; Line 2: The extracts from JK cells ; Line 3: The extracts from K562 cells.
Conjugated Antibody
This product is also available conjugated. Format: E11-0694A-Conjugate. Price: $485 / Size: 100μl.
E11-0694A-FITC$485 / 100μlE11-0694A-Biotin$485 / 100μlE11-0694A-HRP$485 / 100μlE11-0694A-AP$485 / 100μlE11-0694A-AF350$485 / 100μlE11-0694A-AF405M$485 / 100μlE11-0694A-AF488$485 / 100μlE11-0694A-AF514$485 / 100μlE11-0694A-AF532$485 / 100μlE11-0694A-AF555$485 / 100μlE11-0694A-AF568$485 / 100μlE11-0694A-AF405S$485 / 100μlE11-0694A-AF405L$485 / 100μlE11-0694A-AF546$485 / 100μlE11-0694A-AF594$485 / 100μlE11-0694A-AF610$485 / 100μlE11-0694A-AF635$485 / 100μlE11-0694A-AF647$485 / 100μlE11-0694A-AF680$485 / 100μlE11-0694A-AF700$485 / 100μlE11-0694A-AF750$485 / 100μlE11-0694A-AF790$485 / 100μlE11-0694A-APC$485 / 100μlE11-0694A-PE$485 / 100μlE11-0694A-Cy3$485 / 100μlE11-0694A-Cy5$485 / 100μlE11-0694A-Cy5.5$485 / 100μlE11-0694A-Cy7$485 / 100μlE11-0694A-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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