Other Names MLCB, MLRA, MLRM, MRCL3, Myosin RLC, Myosin RLC-A, Myosin regulatory light chain, Myosin regulatory light chain 2, nonsarcomeric, Myosin regulatory light chain 2-A, smooth muscle isoform, RLC, RLC-A
All Sites Human: Ser18; Mouse: Ser20; Rat: Ser19
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Myosin regulatory light chain 2 around the phosphorylation site of serine 18 (A-T-SP-N-V).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Myosin regulatory light chain 2 (Phospho-Ser18) antibody detects endogenous levels of Myosin regulatory light chain 2 only when phosphorylated at serine 18.
ReferencesCarninci P., Science 309:1559-1563(2005).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Lubec G., Submitted (MAR-2007) to UniProtKB.
--------