Other Names FRAP, FRAP1, FRAP2, RAFT1, Rapamycin target protein, kinase mTOR
All Sites Human: Ser2481; Mouse: Ser2481; Rat: Ser2481
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human mTOR around the phosphorylation site of serine 2481 (I-H-SP-F-I).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity mTOR (Phospho-Ser2481) antibody detects endogenous levels of mTOR only when phosphorylated at serine 2481.
ReferencesHajime Hosoi, Cancer Res., Feb 1999; 59: 886 - 894.
P. T. Soliman, ASCO Meeting Abstracts, Jun 2005; 23: 5080.
Megan S. Lim, Blood (ASH Annual Meeting Abstracts), Nov 2006; 108: 290.
Patrick Frost, Blood (ASH Annual Meeting Abstracts), Nov 2004; 104: 646.
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