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Primary Antibodies

MSK1 (Phospho-Thr581) Antibody

E11-0686ACite ID: EN0017399DatasheetCOAMSDS
Human, Mouse
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000
O75582
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names 90 kDa ribosomal protein S6 kinase, , polypeptide 5, EC 2.7.11.1, KS6A5, MSPK1, Nuclear mitogen- and stress-activated protein kinase-1, RLPK, RPS6KA5, kinase MSK1, ribosomal protein S6 kinase, polypeptide V
All Sites Human: Thr581; Mouse: Thr645
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human MSK1 around the phosphorylation site of threonine 581 (L-K-TP-P-C).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity MSK1 (Phospho-Thr581) antibody detects endogenous levels of MSK1 only when phosphorylated at threonine 581.
ReferencesGiselle R. Wiggin, Mol. Cell. Biol., Apr 2002; 22: 2871 - 2881.
Greg Q. Butcher, J. Neurosci., Jun 2005; 25: 5305 - 5313.
Jason A. Gustin, Am J Physiol Cell Physiol, Mar 2004; 286: C547 - C555.
Kyoung Seob Song, J. Biol. Chem., Jun 2003; 278: 23243 - 23250.
Price225$
Western blot analysis of extracts from RAW264.7 cells, treated with UV (5mins), using MSK1 (Phospho-Thr581) antibody.
Western blot analysis of extracts from RAW264.7 cells, treated with UV (5mins), using MSK1 (Phospho-Thr581) antibody.
Immunofluorescence analysis of A549 cells, using MSK1 (Phospho-Thr581) antibody.
Immunofluorescence analysis of A549 cells, using MSK1 (Phospho-Thr581) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0686A-Conjugate. Price: $485 / Size: 100μl.

E11-0686A-FITC$485 / 100μlE11-0686A-Biotin$485 / 100μlE11-0686A-HRP$485 / 100μlE11-0686A-AP$485 / 100μlE11-0686A-AF350$485 / 100μlE11-0686A-AF405M$485 / 100μlE11-0686A-AF488$485 / 100μlE11-0686A-AF514$485 / 100μlE11-0686A-AF532$485 / 100μlE11-0686A-AF555$485 / 100μlE11-0686A-AF568$485 / 100μlE11-0686A-AF405S$485 / 100μlE11-0686A-AF405L$485 / 100μlE11-0686A-AF546$485 / 100μlE11-0686A-AF594$485 / 100μlE11-0686A-AF610$485 / 100μlE11-0686A-AF635$485 / 100μlE11-0686A-AF647$485 / 100μlE11-0686A-AF680$485 / 100μlE11-0686A-AF700$485 / 100μlE11-0686A-AF750$485 / 100μlE11-0686A-AF790$485 / 100μlE11-0686A-APC$485 / 100μlE11-0686A-PE$485 / 100μlE11-0686A-Cy3$485 / 100μlE11-0686A-Cy5$485 / 100μlE11-0686A-Cy5.5$485 / 100μlE11-0686A-Cy7$485 / 100μlE11-0686A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.