Other Names 90 kDa ribosomal protein S6 kinase, , polypeptide 5, EC 2.7.11.1, KS6A5, MSPK1, Nuclear mitogen- and stress-activated protein kinase-1, RLPK, RPS6KA5, kinase MSK1, ribosomal protein S6 kinase, polypeptide V
All Sites Human: Thr581; Mouse: Thr645
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human MSK1 around the phosphorylation site of threonine 581 (L-K-TP-P-C).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity MSK1 (Phospho-Thr581) antibody detects endogenous levels of MSK1 only when phosphorylated at threonine 581.
ReferencesGiselle R. Wiggin, Mol. Cell. Biol., Apr 2002; 22: 2871 - 2881.
Greg Q. Butcher, J. Neurosci., Jun 2005; 25: 5305 - 5313.
Jason A. Gustin, Am J Physiol Cell Physiol, Mar 2004; 286: C547 - C555.
Kyoung Seob Song, J. Biol. Chem., Jun 2003; 278: 23243 - 23250.