Other Names 90 kDa ribosomal protein S6 kinase, , polypeptide 5, EC 2.7.11.1, KS6A5, MSPK1, Nuclear mitogen- and stress-activated protein kinase-1, RLPK, RPS6KA5, kinase MSK1, ribosomal protein S6 kinase, polypeptide V
All Sites Human: Ser360; Mouse: Ser359
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human MSK1 around the phosphorylation site of serine 360 (T-Y-SP-P-A).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity MSK1 (Phospho-Ser360) antibody detects endogenous levels of MSK1 only when phosphorylated at serine 360.
ReferencesDeak M., EMBO J. 17:4426-4441(1998).
New L., J. Biol. Chem. 274:1026-1032(1999).
Zhang Y., J. Biol. Chem. 279:21866-21872(2004).