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Primary Antibodies

MEK1 (Phospho-Thr286) Antibody

E11-0681ACite ID: EN0017395DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
Q02750
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names Dual specificity mitogen-activated protein kinase kinase 1, EC 2.7.12.2, ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK1, MP2K1, PRKMK1, kinase MEK1
All Sites Human: Thr286; Mouse: Thr286; Rat: Thr286
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human MEK1 around the phosphorylation site of threonine 286 (A-E-TP-P-P).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity MEK1 (Phospho-Thr286) antibody detects endogenous levels of MEK1 only when phosphorylated at threonine 286.
ReferencesShunichi Murakami, Genes & Dev., Feb 2004; 18: 290.
Ronald A. Rimerman, J. Biol. Chem., May 2000; 275: 14736.
Anne C. Marinovic, J. Biol. Chem., May 2002; 277: 16673 - 16681.
Rohit Lal, AACR Meeting Abstracts, Apr 2006; 2006: 687.
Price225$
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MEK1 (Phospho- Thr286) antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MEK1 (Phospho- Thr286) antibody.
Western blot analysis of extracts from K562 cells, using MEK1 (Phospho-Thr286) antibody.
Western blot analysis of extracts from K562 cells, using MEK1 (Phospho-Thr286) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0681A-Conjugate. Price: $485 / Size: 100μl.

E11-0681A-FITC$485 / 100μlE11-0681A-Biotin$485 / 100μlE11-0681A-HRP$485 / 100μlE11-0681A-AP$485 / 100μlE11-0681A-AF350$485 / 100μlE11-0681A-AF405M$485 / 100μlE11-0681A-AF488$485 / 100μlE11-0681A-AF514$485 / 100μlE11-0681A-AF532$485 / 100μlE11-0681A-AF555$485 / 100μlE11-0681A-AF568$485 / 100μlE11-0681A-AF405S$485 / 100μlE11-0681A-AF405L$485 / 100μlE11-0681A-AF546$485 / 100μlE11-0681A-AF594$485 / 100μlE11-0681A-AF610$485 / 100μlE11-0681A-AF635$485 / 100μlE11-0681A-AF647$485 / 100μlE11-0681A-AF680$485 / 100μlE11-0681A-AF700$485 / 100μlE11-0681A-AF750$485 / 100μlE11-0681A-AF790$485 / 100μlE11-0681A-APC$485 / 100μlE11-0681A-PE$485 / 100μlE11-0681A-Cy3$485 / 100μlE11-0681A-Cy5$485 / 100μlE11-0681A-Cy5.5$485 / 100μlE11-0681A-Cy7$485 / 100μlE11-0681A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.