Other Names Dual specificity mitogen-activated protein kinase kinase 1, EC 2.7.12.2, ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK1, MP2K1, PRKMK1, kinase MEK1
All Sites Human: Thr286; Mouse: Thr286; Rat: Thr286
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human MEK1 around the phosphorylation site of threonine 286 (A-E-TP-P-P).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity MEK1 (Phospho-Thr286) antibody detects endogenous levels of MEK1 only when phosphorylated at threonine 286.
ReferencesShunichi Murakami, Genes & Dev., Feb 2004; 18: 290.
Ronald A. Rimerman, J. Biol. Chem., May 2000; 275: 14736.
Anne C. Marinovic, J. Biol. Chem., May 2002; 277: 16673 - 16681.
Rohit Lal, AACR Meeting Abstracts, Apr 2006; 2006: 687.