Other Names Dual specificity mitogen-activated protein kinase kinase 1; EC 2.7.12.2; ERK activator kinase 1; kinase MEK1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK1; MP2K1; PRKMK1
All Sites Human: Ser298; Mouse: Ser298; Rat: Ser298
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human MEK1 around the phosphorylation site of serine 298(P-L-SP-S-Y).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity MEK1 (Phospho-Ser298) antibody detects endogenous levels of MEK1 only when phosphorylated at serine 298.
ReferencesSeger R., J. Biol. Chem. 267:25628-25631(1992).
Zheng C.-F., J. Biol. Chem. 268:11435-11439(1993).
Zheng C.-F., EMBO J. 13:1123-1131(1994)