World supplier of antibodies, antigens and kits since 2007
Primary Antibodies
Histone H3 (Phospho-Thr11) Antibody
Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)
IF: 1:500~1:1000 ELISA: 1:1000
$225/ 100μg
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Other Names H3/a, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l, H31, HIST1H3A, histone H3.1
All Sites Human: Thr11; Mouse: Thr11; Rat: Thr12
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Histone H3 around the phosphorylation site of threonine 11 (K-S-TP-G-G).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Histone H3 (Phospho-Thr11) antibody detects endogenous levels of Histone H3 only when phosphorylated at threonine 11.
ReferencesKyu Heo. J. Biol. Chem., May 2007; 282: 15476 - 15483.
Sandra B. Hake. PNAS, Apr 2006; 103: 6428 - 6435.
Jeong-Heon Lee. J. Biol. Chem., May 2007; 282: 13419 - 13428.
Sandra B. Hake. PNAS, Apr 2006; 103: 6428 - 6435.
Jeong-Heon Lee. J. Biol. Chem., May 2007; 282: 13419 - 13428.
Price225$
Immunofluorescence analysis of HuvEc cells, using Histone H3 (Phospho-Thr11) antibody.
Histone H3 (Phospho-Thr11) antibody reacts with epitope- specific phosphopeptide and corresponding non- phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.
Conjugated Antibody
This product is also available conjugated. Format: E11-0662A-Conjugate. Price: $485 / Size: 100μl.
E11-0662A-FITC$485 / 100μlE11-0662A-Biotin$485 / 100μlE11-0662A-HRP$485 / 100μlE11-0662A-AP$485 / 100μlE11-0662A-AF350$485 / 100μlE11-0662A-AF405M$485 / 100μlE11-0662A-AF488$485 / 100μlE11-0662A-AF514$485 / 100μlE11-0662A-AF532$485 / 100μlE11-0662A-AF555$485 / 100μlE11-0662A-AF568$485 / 100μlE11-0662A-AF405S$485 / 100μlE11-0662A-AF405L$485 / 100μlE11-0662A-AF546$485 / 100μlE11-0662A-AF594$485 / 100μlE11-0662A-AF610$485 / 100μlE11-0662A-AF635$485 / 100μlE11-0662A-AF647$485 / 100μlE11-0662A-AF680$485 / 100μlE11-0662A-AF700$485 / 100μlE11-0662A-AF750$485 / 100μlE11-0662A-AF790$485 / 100μlE11-0662A-APC$485 / 100μlE11-0662A-PE$485 / 100μlE11-0662A-Cy3$485 / 100μlE11-0662A-Cy5$485 / 100μlE11-0662A-Cy5.5$485 / 100μlE11-0662A-Cy7$485 / 100μlE11-0662A-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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