Other Names EIF4G1, EIF4GI, Eukaryotic translation initiation factor 4GI, IF4G1, eIF-4-gamma 1, eIF-4G, eIF-4G1, eukaryotic translation initiation factor 4 gamma 1, p220
All Sites Human: Ser1108; Mouse: Ser1149; Rat: Ser950
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Eif4g around the phosphorylation site of serine 1108 (S-L-SP-R-E).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Eif4g (Phospho-Ser1108) antibody detects endogenous levels of Eif4g only when phosphorylated at serine 1108.
ReferencesYan R., J. Biol. Chem. 267:23226-23231(1992).
Imataka H., Mol. Cell. Biol. 17:6940-6947(1997).
Byrd M.P., Mol. Cell. Biol. 22:4499-4511(2002).