Other Names CCNE, G1/S-specific cyclin E1, cyclin E1
All Sites Human: Thr77; Mouse: Thr74
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Cyclin E1 around the phosphorylation site of threonine 77 (I-P-TP-P-D).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Cyclin E1 (Phospho-Thr77) antibody detects endogenous levels of Cyclin E1 only when phosphorylated at threonine 77.
ReferencesKoff A., Cell 66:1217-1228(1991).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Geng Y., Oncogene 12:1173-1180(1996).