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Primary Antibodies

Cyclin B1 (Phospho-Ser147) Antibody

E11-0635ACite ID: EN0017362DatasheetCOAMSDS
Human, Mouse
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
P14635
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names CCN-2, CCNB, CCNB1, CCNB1-RS1, CYCB, CYCB1, G2/mitotic-specific cyclin B1
All Sites Human: Ser147; Mouse: Ser144
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Cyclin B1 around the phosphorylation site of serine 147 (A-F-SP-D-V).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity Cyclin B1 (Phospho-Ser147) antibody detects endogenous levels of Cyclin B1 only when phosphorylated at serine 147.
ReferencesCatherine A. Rugg, AACR Meeting Abstracts, Apr 2005; 2005: 1047.
Jianping Gong, Cancer Res., Aug 1994; 54: 4285 - 4288.
Lourdes A. Gomez, Mol. Cancer Ther., May 2007; 6: 1534 - 1543.
Clare E. Davy, J. Virol., Apr 2005; 79: 3998 - 4011.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemistry analysis of paraffin-embedded human placenta tissue using Cyclin B1 (Phospho-Ser147) antibody.
Immunohistochemistry analysis of paraffin-embedded human placenta tissue using Cyclin B1 (Phospho-Ser147) antibody.
Western blot analysis of extracts from Jurkat cells, treated with UV (15mins), using Cyclin B1 (Phospho-Ser147) antibody.
Western blot analysis of extracts from Jurkat cells, treated with UV (15mins), using Cyclin B1 (Phospho-Ser147) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0635A-Conjugate. Price: $485 / Size: 100μl.

E11-0635A-FITC$485 / 100μlE11-0635A-Biotin$485 / 100μlE11-0635A-HRP$485 / 100μlE11-0635A-AP$485 / 100μlE11-0635A-AF350$485 / 100μlE11-0635A-AF405M$485 / 100μlE11-0635A-AF488$485 / 100μlE11-0635A-AF514$485 / 100μlE11-0635A-AF532$485 / 100μlE11-0635A-AF555$485 / 100μlE11-0635A-AF568$485 / 100μlE11-0635A-AF405S$485 / 100μlE11-0635A-AF405L$485 / 100μlE11-0635A-AF546$485 / 100μlE11-0635A-AF594$485 / 100μlE11-0635A-AF610$485 / 100μlE11-0635A-AF635$485 / 100μlE11-0635A-AF647$485 / 100μlE11-0635A-AF680$485 / 100μlE11-0635A-AF700$485 / 100μlE11-0635A-AF750$485 / 100μlE11-0635A-AF790$485 / 100μlE11-0635A-APC$485 / 100μlE11-0635A-PE$485 / 100μlE11-0635A-Cy3$485 / 100μlE11-0635A-Cy5$485 / 100μlE11-0635A-Cy5.5$485 / 100μlE11-0635A-Cy7$485 / 100μlE11-0635A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.