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Primary Antibodies
c-Raf (Phospho-Ser296) Antibody
$225/ 100μg
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Other Names C-RAF, C-Raf, CRAF, EC 2.7.11.1, Protein kinase raf 1, RAF proto-oncogene serine/threonine-protein kinase, RAF-1, RAF1, Raf proto-oncogene serine/threonine-protein kinase, Raf-1, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1, kinase Raf1
All Sites Human: Ser296; Mouse: Ser296; Rat: Ser296
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human c-Raf around the phosphorylation site of serine 296 (S-S-SP-P-N).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity c-Raf (Phospho-Ser296) antibody detects endogenous levels of c-Raf only when phosphorylated at serine 296.
ReferencesMarie A. Bogoyevitch, J. Biol. Chem., Nov 1995; 270: 26303.
G Heidecker, Mol. Cell. Biol., Jun 1990; 10: 2503 - 2512.
Shari L. Sutor, J. Biol. Chem., Mar 1999; 274: 7002.
Albrecht Piiper, Mol. Pharmacol., Sep 2000; 58: 608.
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G Heidecker, Mol. Cell. Biol., Jun 1990; 10: 2503 - 2512.
Shari L. Sutor, J. Biol. Chem., Mar 1999; 274: 7002.
Albrecht Piiper, Mol. Pharmacol., Sep 2000; 58: 608.
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Price225$
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using c-Raf (Phospho- Ser296) antibody.
Western blot analysis of extracts from 293 cells, treated with PMA (125ng/ml, 30mins), using c-Raf (Phospho- Ser296) antibody.
Conjugated Antibody
This product is also available conjugated. Format: E11-0634A-Conjugate. Price: $485 / Size: 100μl.
E11-0634A-FITC$485 / 100μlE11-0634A-Biotin$485 / 100μlE11-0634A-HRP$485 / 100μlE11-0634A-AP$485 / 100μlE11-0634A-AF350$485 / 100μlE11-0634A-AF405M$485 / 100μlE11-0634A-AF488$485 / 100μlE11-0634A-AF514$485 / 100μlE11-0634A-AF532$485 / 100μlE11-0634A-AF555$485 / 100μlE11-0634A-AF568$485 / 100μlE11-0634A-AF405S$485 / 100μlE11-0634A-AF405L$485 / 100μlE11-0634A-AF546$485 / 100μlE11-0634A-AF594$485 / 100μlE11-0634A-AF610$485 / 100μlE11-0634A-AF635$485 / 100μlE11-0634A-AF647$485 / 100μlE11-0634A-AF680$485 / 100μlE11-0634A-AF700$485 / 100μlE11-0634A-AF750$485 / 100μlE11-0634A-AF790$485 / 100μlE11-0634A-APC$485 / 100μlE11-0634A-PE$485 / 100μlE11-0634A-Cy3$485 / 100μlE11-0634A-Cy5$485 / 100μlE11-0634A-Cy5.5$485 / 100μlE11-0634A-Cy7$485 / 100μlE11-0634A-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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