Other Names CDC25M1, Dual specificity phosphatase Cdc25C, EC 3.1.3.48, M-phase inducer phosphatase 3, MPIP3
All Sites Human: Thr48; Mouse: Thr48
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human CDC25C around the phosphorylation site of threonine 48 (P-R-TP-P-V).
Purification The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity CDC25C (Phospho-Thr48) antibody detects endogenous levels of CDC25C only when phosphorylated at threonine 48.
ReferencesBureik M., Int. J. Oncol. 17:1251-1258(2000).
Hernandez S., Lab. Invest. 81:465-473(2001).
Beausoleil S.A., Nat. Biotechnol. 24:1285-1292(2006).