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Primary Antibodies

Akt (Phospho-Thr72) Antibody

E11-0610ACite ID: EN0017340DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
P31749
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names AKT, AKT1 kinase, C-AKT, EC 2.7.11.1, PKB, PKB-alpha, Protein kinase B, RAC, RAC-PK-alpha, RAC-alpha serine/threonine kinase, kinase Akt1
All Sites Human: Thr72; Mouse: Thr72; Rat: Thr72
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human AKT around the phosphorylation site of threonine 72 (P-N-TP-F-I).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity Akt (Phospho-Thr72) antibody detects endogenous levels of AKT only when phosphorylated at threonine 72.
ReferencesKirsten H. Limesand. Mol. Cell. Biol., Dec 2006; 26: 8840 - 8856.
Brian T. O’Neill. J. Clin. Invest., Aug 2005; 115: 2059 - 2064. V
Zhong-Zhou Yang. J. Biol. Chem., Aug 2003; 278: 32124 - 32131.
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Price225$
Immunohistochemistry analysis of paraffin-embedded human ovary tissue using Akt (Phospho-Thr72) antibody.
Immunohistochemistry analysis of paraffin-embedded human ovary tissue using Akt (Phospho-Thr72) antibody.
Western blot analysis of extracts from NIH/3T3cells, treated with TNF-a (20ng/ml, 30mins), using Akt (Phospho-Thr72) antibody.
Western blot analysis of extracts from NIH/3T3cells, treated with TNF-a (20ng/ml, 30mins), using Akt (Phospho-Thr72) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0610A-Conjugate. Price: $485 / Size: 100μl.

E11-0610A-FITC$485 / 100μlE11-0610A-Biotin$485 / 100μlE11-0610A-HRP$485 / 100μlE11-0610A-AP$485 / 100μlE11-0610A-AF350$485 / 100μlE11-0610A-AF405M$485 / 100μlE11-0610A-AF488$485 / 100μlE11-0610A-AF514$485 / 100μlE11-0610A-AF532$485 / 100μlE11-0610A-AF555$485 / 100μlE11-0610A-AF568$485 / 100μlE11-0610A-AF405S$485 / 100μlE11-0610A-AF405L$485 / 100μlE11-0610A-AF546$485 / 100μlE11-0610A-AF594$485 / 100μlE11-0610A-AF610$485 / 100μlE11-0610A-AF635$485 / 100μlE11-0610A-AF647$485 / 100μlE11-0610A-AF680$485 / 100μlE11-0610A-AF700$485 / 100μlE11-0610A-AF750$485 / 100μlE11-0610A-AF790$485 / 100μlE11-0610A-APC$485 / 100μlE11-0610A-PE$485 / 100μlE11-0610A-Cy3$485 / 100μlE11-0610A-Cy5$485 / 100μlE11-0610A-Cy5.5$485 / 100μlE11-0610A-Cy7$485 / 100μlE11-0610A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.