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Primary Antibodies
Akt (Phospho-Ser129) Antibody
Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)
WB: 1:500~1:1000 ELISA: 1:1000
$225/ 100μg
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Other Names AKT; AKT1 kinase; C-AKT; EC 2.7.11.1; kinase Akt1; PKB; PKB-alpha; Protein kinase B; RAC; RAC-alpha serine/threonine kinase; RAC-PK-alpha
All Sites Human: Ser129; Mouse: Ser129; Rat: Ser129
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of serine 129 (D-N-SP-G-A).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Akt (Phospho-Ser129) antibody detects endogenous levels of Akt only when phosphorylated at serine 129.
ReferencesJones P.F., Proc. Natl. Acad. Sci. U.S.A. 88:4171-4175(1991).
Matsubara A., Diabetologia 44:910-913(2001).
The MGC Project Team; Genome Res. 14-2121-2127(2004).
Matsubara A., Diabetologia 44:910-913(2001).
The MGC Project Team; Genome Res. 14-2121-2127(2004).
Price225$
Western blot analysis of extracts from A549 cells, treated with PMA (125ng/ml, 30mins), using Akt (Phospho-Ser129) antibody.
Conjugated Antibody
This product is also available conjugated. Format: E11-0609A-Conjugate. Price: $485 / Size: 100μl.
E11-0609A-FITC$485 / 100μlE11-0609A-Biotin$485 / 100μlE11-0609A-HRP$485 / 100μlE11-0609A-AP$485 / 100μlE11-0609A-AF350$485 / 100μlE11-0609A-AF405M$485 / 100μlE11-0609A-AF488$485 / 100μlE11-0609A-AF514$485 / 100μlE11-0609A-AF532$485 / 100μlE11-0609A-AF555$485 / 100μlE11-0609A-AF568$485 / 100μlE11-0609A-AF405S$485 / 100μlE11-0609A-AF405L$485 / 100μlE11-0609A-AF546$485 / 100μlE11-0609A-AF594$485 / 100μlE11-0609A-AF610$485 / 100μlE11-0609A-AF635$485 / 100μlE11-0609A-AF647$485 / 100μlE11-0609A-AF680$485 / 100μlE11-0609A-AF700$485 / 100μlE11-0609A-AF750$485 / 100μlE11-0609A-AF790$485 / 100μlE11-0609A-APC$485 / 100μlE11-0609A-PE$485 / 100μlE11-0609A-Cy3$485 / 100μlE11-0609A-Cy5$485 / 100μlE11-0609A-Cy5.5$485 / 100μlE11-0609A-Cy7$485 / 100μlE11-0609A-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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