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Primary Antibodies

ACK1 (Phospho-Tyr284) Antibody

E11-0604ACite ID: EN0017331DatasheetCOAMSDS
Human, Mouse
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000
Q07912
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names ACK1, Activated p21cdc42Hs kinase, EC 2.7.10.2, Non-receptor protein tyrosine kinase Ack, TNK2, kinase ACK1
All Sites Human: Tyr284; Mouse: Tyr284
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human ACK1 around the phosphorylation site of tyrosine 284 (D-H-YP-V-M).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity ACK1 (Phospho-Tyr284) antibody detects endogenous levels of ACK1 only when phosphorylated at tyrosine 284.
ReferencesManser E., Nature 363:364-367(1993).
Ota T., Nat. Genet. 36:40-45(2004).
Eisenmann K.M., Nat. Cell Biol. 1:507-513(1999).
Price225$
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ACK1 (Phospho- Tyr284) antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ACK1 (Phospho- Tyr284) antibody.
Western blot analysis of extracts from HepG2 cells, treated with EGF (200ng/ml, 30mins), using ACK1 (Phospho-Tyr284) antibody.
Western blot analysis of extracts from HepG2 cells, treated with EGF (200ng/ml, 30mins), using ACK1 (Phospho-Tyr284) antibody.
Immunofluorescence analysis of A549 cells, using ACK1 (Phospho-Tyr284) antibody.
Immunofluorescence analysis of A549 cells, using ACK1 (Phospho-Tyr284) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0604A-Conjugate. Price: $485 / Size: 100μl.

E11-0604A-FITC$485 / 100μlE11-0604A-Biotin$485 / 100μlE11-0604A-HRP$485 / 100μlE11-0604A-AP$485 / 100μlE11-0604A-AF350$485 / 100μlE11-0604A-AF405M$485 / 100μlE11-0604A-AF488$485 / 100μlE11-0604A-AF514$485 / 100μlE11-0604A-AF532$485 / 100μlE11-0604A-AF555$485 / 100μlE11-0604A-AF568$485 / 100μlE11-0604A-AF405S$485 / 100μlE11-0604A-AF405L$485 / 100μlE11-0604A-AF546$485 / 100μlE11-0604A-AF594$485 / 100μlE11-0604A-AF610$485 / 100μlE11-0604A-AF635$485 / 100μlE11-0604A-AF647$485 / 100μlE11-0604A-AF680$485 / 100μlE11-0604A-AF700$485 / 100μlE11-0604A-AF750$485 / 100μlE11-0604A-AF790$485 / 100μlE11-0604A-APC$485 / 100μlE11-0604A-PE$485 / 100μlE11-0604A-Cy3$485 / 100μlE11-0604A-Cy5$485 / 100μlE11-0604A-Cy5.5$485 / 100μlE11-0604A-Cy7$485 / 100μlE11-0604A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.