Other Names ACK1, Activated p21cdc42Hs kinase, EC 2.7.10.2, Non-receptor protein tyrosine kinase Ack, TNK2, kinase ACK1
All Sites Human: Tyr284; Mouse: Tyr284
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human ACK1 around the phosphorylation site of tyrosine 284 (D-H-YP-V-M).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity ACK1 (Phospho-Tyr284) antibody detects endogenous levels of ACK1 only when phosphorylated at tyrosine 284.
ReferencesManser E., Nature 363:364-367(1993).
Ota T., Nat. Genet. 36:40-45(2004).
Eisenmann K.M., Nat. Cell Biol. 1:507-513(1999).