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Primary Antibodies

ZAP-70 (Phospho-Tyr315) Antibody

E11-0601ACite ID: EN0017329DatasheetCOAMSDS
Human, Mouse
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
P43403
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names 70 kDa zeta-associated protein, EC 2.7.10.2, SRK, Syk-related tyrosine kinase, ZA70, ZAP-70, ZAP70, kinase ZAP70
All Sites Human: Ser315; Mouse: Ser314
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human ZAP-70 around the phosphorylation site of tyrosine 315 (S-V-YP-E-S).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity ZAP-70 (Phospho-Tyr315) antibody detects endogenous levels of ZAP-70 only when phosphorylated at tyrosine 315.
ReferencesDL Levey, J. Exp. Med., Oct 1995; 182: 1029.
Liguang Chen, Blood, Mar 2005; 105: 2036 - 2041.
Adrian Wiestner, Blood, Jun 2003; 101: 4944 - 4951.
Makoto Otsu, Blood, Jul 2002; 100: 1248 - 1256.
Price225$
Western blot analysis of extracts from Jurkat cells, treated with Ca+ (40nM, 30mins), using ZAP-70 (Phospho- Tyr315) antibody.
Western blot analysis of extracts from Jurkat cells, treated with Ca+ (40nM, 30mins), using ZAP-70 (Phospho- Tyr315) antibody.
📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E11-0601A-Conjugate. Price: $485 / Size: 100μl.

E11-0601A-FITC$485 / 100μlE11-0601A-Biotin$485 / 100μlE11-0601A-HRP$485 / 100μlE11-0601A-AP$485 / 100μlE11-0601A-AF350$485 / 100μlE11-0601A-AF405M$485 / 100μlE11-0601A-AF488$485 / 100μlE11-0601A-AF514$485 / 100μlE11-0601A-AF532$485 / 100μlE11-0601A-AF555$485 / 100μlE11-0601A-AF568$485 / 100μlE11-0601A-AF405S$485 / 100μlE11-0601A-AF405L$485 / 100μlE11-0601A-AF546$485 / 100μlE11-0601A-AF594$485 / 100μlE11-0601A-AF610$485 / 100μlE11-0601A-AF635$485 / 100μlE11-0601A-AF647$485 / 100μlE11-0601A-AF680$485 / 100μlE11-0601A-AF700$485 / 100μlE11-0601A-AF750$485 / 100μlE11-0601A-AF790$485 / 100μlE11-0601A-APC$485 / 100μlE11-0601A-PE$485 / 100μlE11-0601A-Cy3$485 / 100μlE11-0601A-Cy5$485 / 100μlE11-0601A-Cy5.5$485 / 100μlE11-0601A-Cy7$485 / 100μlE11-0601A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.

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