Other Names 70 kDa zeta-associated protein, EC 2.7.10.2, SRK, Syk-related tyrosine kinase, ZA70, ZAP-70, ZAP70, kinase ZAP70
All Sites Human: Tyr292; Mouse: Tyr290
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human ZAP-70 around the phosphorylation site of tyrosine 292 (D-G-YP-T-P).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity ZAP-70 (Phospho-Tyr292) antibody detects endogenous levels of ZAP-70 only when phosphorylated at tyrosine 292.
ReferencesDL Levey, J. Exp. Med., Oct 1995; 182: 1029.
Liguang Chen, Blood, Mar 2005; 105: 2036 - 2041.
Adrian Wiestner, Blood, Jun 2003; 101: 4944 - 4951.
Makoto Otsu, Blood, Jul 2002; 100: 1248 - 1256.