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Primary Antibodies

C-RAF (Phospho-Ser621) Antibody

E11-0566ACite ID: EN0017269DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000
P04049
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names C-RAF, C-Raf, CRAF, EC 2.7.11.1, Protein kinase raf 1, RAF proto-oncogene serine/threonine-protein kinase, RAF-1, RAF1, Raf proto-oncogene serine/threonine-protein kinase, Raf-1, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1, kinase Raf1
All Sites Human: Ser621; Mouse: Ser621; Rat: Ser621
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human RAF1 around the phosphorylation site of serine 621 (S-A-SP-E-P).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity Raf1 (Phospho-Ser621) antibody detects endogenous levels of RAF1 only when phosphorylated at serine 621.
ReferencesDavid R. Hodge, J. Biol. Chem., Jun 1998; 273: 15727.
Michael A. Beazely, Mol. Pharmacol., Jan 2005; 67: 250 - 259.
Deanna G. Adams, J. Biol. Chem., Dec 2005; 280: 42644 - 42654.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Raf1 (Phospho- Ser621) antibody.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Raf1 (Phospho- Ser621) antibody.
Western blot analysis of extracts from HeLa cells, treated with UV (5mins), using Raf1 (Phospho-Ser621) antibody.
Western blot analysis of extracts from HeLa cells, treated with UV (5mins), using Raf1 (Phospho-Ser621) antibody.
Immunofluorescence analysis of HeLa cells, using Raf1 (Phospho-Ser621) antibody.
Immunofluorescence analysis of HeLa cells, using Raf1 (Phospho-Ser621) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0566A-Conjugate. Price: $485 / Size: 100μl.

E11-0566A-FITC$485 / 100μlE11-0566A-Biotin$485 / 100μlE11-0566A-HRP$485 / 100μlE11-0566A-AP$485 / 100μlE11-0566A-AF350$485 / 100μlE11-0566A-AF405M$485 / 100μlE11-0566A-AF488$485 / 100μlE11-0566A-AF514$485 / 100μlE11-0566A-AF532$485 / 100μlE11-0566A-AF555$485 / 100μlE11-0566A-AF568$485 / 100μlE11-0566A-AF405S$485 / 100μlE11-0566A-AF405L$485 / 100μlE11-0566A-AF546$485 / 100μlE11-0566A-AF594$485 / 100μlE11-0566A-AF610$485 / 100μlE11-0566A-AF635$485 / 100μlE11-0566A-AF647$485 / 100μlE11-0566A-AF680$485 / 100μlE11-0566A-AF700$485 / 100μlE11-0566A-AF750$485 / 100μlE11-0566A-AF790$485 / 100μlE11-0566A-APC$485 / 100μlE11-0566A-PE$485 / 100μlE11-0566A-Cy3$485 / 100μlE11-0566A-Cy5$485 / 100μlE11-0566A-Cy5.5$485 / 100μlE11-0566A-Cy7$485 / 100μlE11-0566A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.