Other Names C-RAF, C-Raf, CRAF, EC 2.7.11.1, Protein kinase raf 1, RAF proto-oncogene serine/threonine-protein kinase, RAF-1, RAF1, Raf proto-oncogene serine/threonine-protein kinase, Raf-1, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1, kinase Raf1
All Sites Human: Ser621; Mouse: Ser621; Rat: Ser621
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human RAF1 around the phosphorylation site of serine 621 (S-A-SP-E-P).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Raf1 (Phospho-Ser621) antibody detects endogenous levels of RAF1 only when phosphorylated at serine 621.
ReferencesDavid R. Hodge, J. Biol. Chem., Jun 1998; 273: 15727.
Michael A. Beazely, Mol. Pharmacol., Jan 2005; 67: 250 - 259.
Deanna G. Adams, J. Biol. Chem., Dec 2005; 280: 42644 - 42654.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells