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Primary Antibodies

PKA α/β CAT (Phospho-Thr197) Antibody

E11-0548ACite ID: EN0017241DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000
P17612
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; PRKACA ;PKACA ; cAMP-dependent protein kinase catalytic subunit beta; PKA C-beta; PRKACB
All Sites Human: Thr197; Mouse: Thr197; Rat: Thr197
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PKA α/β CAT around the phosphorylation site of threonine 197 (T-W-TP-L-C).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity PKA α/β CAT (Phospho-Thr197) antibody detects endogenous levels of PKA α/β CAT only when phosphorylated at threonine 197.
ReferencesGhislaine Garrel, Endocrinology, Jun 1997; 138: 2259.
W Li, Learn. Mem., Jan 1996; 2: 320 - 333.
Sharmin Schauble, J. Biol. Chem., May 2007; 282: 14952 - 14959.
Yong G. Wang, J. Gen. Physiol., Jan 1998; 111: 113.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using PKA α/β (Phospho-Thr197) antibody.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using PKA α/β (Phospho-Thr197) antibody.
Western blot analysis of extracts from CAT mouse brain cells, using PKA α/β CAT (Phospho-Thr197) antibody.
Western blot analysis of extracts from CAT mouse brain cells, using PKA α/β CAT (Phospho-Thr197) antibody.
Immunofluorescence analysis of A549 cells, using PKA α/β CAT (Phospho-Thr197) antibody.
Immunofluorescence analysis of A549 cells, using PKA α/β CAT (Phospho-Thr197) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0548A-Conjugate. Price: $485 / Size: 100μl.

E11-0548A-FITC$485 / 100μlE11-0548A-Biotin$485 / 100μlE11-0548A-HRP$485 / 100μlE11-0548A-AP$485 / 100μlE11-0548A-AF350$485 / 100μlE11-0548A-AF405M$485 / 100μlE11-0548A-AF488$485 / 100μlE11-0548A-AF514$485 / 100μlE11-0548A-AF532$485 / 100μlE11-0548A-AF555$485 / 100μlE11-0548A-AF568$485 / 100μlE11-0548A-AF405S$485 / 100μlE11-0548A-AF405L$485 / 100μlE11-0548A-AF546$485 / 100μlE11-0548A-AF594$485 / 100μlE11-0548A-AF610$485 / 100μlE11-0548A-AF635$485 / 100μlE11-0548A-AF647$485 / 100μlE11-0548A-AF680$485 / 100μlE11-0548A-AF700$485 / 100μlE11-0548A-AF750$485 / 100μlE11-0548A-AF790$485 / 100μlE11-0548A-APC$485 / 100μlE11-0548A-PE$485 / 100μlE11-0548A-Cy3$485 / 100μlE11-0548A-Cy5$485 / 100μlE11-0548A-Cy5.5$485 / 100μlE11-0548A-Cy7$485 / 100μlE11-0548A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.