Other Names cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; PRKACA ;PKACA ; cAMP-dependent protein kinase catalytic subunit beta; PKA C-beta; PRKACB
All Sites Human: Thr197; Mouse: Thr197; Rat: Thr197
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PKA α/β CAT around the phosphorylation site of threonine 197 (T-W-TP-L-C).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity PKA α/β CAT (Phospho-Thr197) antibody detects endogenous levels of PKA α/β CAT only when phosphorylated at threonine 197.
ReferencesGhislaine Garrel, Endocrinology, Jun 1997; 138: 2259.
W Li, Learn. Mem., Jan 1996; 2: 320 - 333.
Sharmin Schauble, J. Biol. Chem., May 2007; 282: 14952 - 14959.
Yong G. Wang, J. Gen. Physiol., Jan 1998; 111: 113.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells