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Primary Antibodies

PDE4D (Phospho-Ser190/53) Antibody

E11-0543ACite ID: EN0017234DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Q08499
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names DPDE3, EC 3.1.4.17, PDE43, PDE4D4, cAMP-specific 3', 5'-cyclic phosphodiesterase 4D
All Sites Human: Ser190; Mouse: Ser129; Rat: Ser185
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PDE4D around the phosphorylation site of serine 190/53 (R-E-SP-F-L).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity PDE4D (Phospho-Ser190/53) antibody detects endogenous levels of PDE4D only when phosphorylated at serine 190/53.
ReferencesSimon J. MacKenzie, J. Biol. Chem., May 2000; 275: 16609.
CÉLINE MÉHATS, FASEB J, Oct 2003; 17: 1831.
Guénaëlle Levallet, Biol Reprod, May 2007; 76: 794 - 803.
Daniel Peter, J. Immunol., Apr 2007; 178: 4820 - 4831.
Price225$
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using (Phospho-Ser190/53) antibody.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using (Phospho-Ser190/53) antibody.
Western blot analysis of extracts from K562 PDE4D cells, treated with H 2 O 2 (100uM, 30mins), using PDE4D (Phospho-Ser190/53) antibody.
Western blot analysis of extracts from K562 PDE4D cells, treated with H 2 O 2 (100uM, 30mins), using PDE4D (Phospho-Ser190/53) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0543A-Conjugate. Price: $485 / Size: 100μl.

E11-0543A-FITC$485 / 100μlE11-0543A-Biotin$485 / 100μlE11-0543A-HRP$485 / 100μlE11-0543A-AP$485 / 100μlE11-0543A-AF350$485 / 100μlE11-0543A-AF405M$485 / 100μlE11-0543A-AF488$485 / 100μlE11-0543A-AF514$485 / 100μlE11-0543A-AF532$485 / 100μlE11-0543A-AF555$485 / 100μlE11-0543A-AF568$485 / 100μlE11-0543A-AF405S$485 / 100μlE11-0543A-AF405L$485 / 100μlE11-0543A-AF546$485 / 100μlE11-0543A-AF594$485 / 100μlE11-0543A-AF610$485 / 100μlE11-0543A-AF635$485 / 100μlE11-0543A-AF647$485 / 100μlE11-0543A-AF680$485 / 100μlE11-0543A-AF700$485 / 100μlE11-0543A-AF750$485 / 100μlE11-0543A-AF790$485 / 100μlE11-0543A-APC$485 / 100μlE11-0543A-PE$485 / 100μlE11-0543A-Cy3$485 / 100μlE11-0543A-Cy5$485 / 100μlE11-0543A-Cy5.5$485 / 100μlE11-0543A-Cy7$485 / 100μlE11-0543A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.

PDE4D (Phospho-Ser190/53) Antibody | EnoGene Biotech Co. Ltd