Other Names DPDE3, EC 3.1.4.17, PDE43, PDE4D4, cAMP-specific 3', 5'-cyclic phosphodiesterase 4D
All Sites Human: Ser190; Mouse: Ser129; Rat: Ser185
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PDE4D around the phosphorylation site of serine 190/53 (R-E-SP-F-L).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity PDE4D (Phospho-Ser190/53) antibody detects endogenous levels of PDE4D only when phosphorylated at serine 190/53.
ReferencesSimon J. MacKenzie, J. Biol. Chem., May 2000; 275: 16609.
CÉLINE MÉHATS, FASEB J, Oct 2003; 17: 1831.
Guénaëlle Levallet, Biol Reprod, May 2007; 76: 794 - 803.
Daniel Peter, J. Immunol., Apr 2007; 178: 4820 - 4831.