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Primary Antibodies

p53 (Phospho-Ser392) Antibody

E11-0530ACite ID: EN0017211DatasheetCOAMSDS
Human
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000
P04637
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names Antigen NY-CO-13, Cellular tumor antigen p53, Phosphoprotein p53, TP53, Tumor suppressor p53
All Sites Human: Ser392
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human p53 around the phosphorylation site of serine 392 (E-G-P-D-SP).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity p53 (Phospho-Ser392) antibody detects endogenous levels of p53 only when phosphorylated at serine 392.
ReferencesLloyd M. Alderson, Cancer Res., Mar 1995; 55: 999 - 1001.
WE Mercer, PNAS, Aug 1990; 87: 6166.
Timothy J. Underwood, AACR Meeting Abstracts, Apr 2006; 2006: 1167.
Jean-Christophe Bourdon, Genes & Dev., Sep 2005; 19: 2122 - 2137.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using p53 (Phospho- Ser392) antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using p53 (Phospho- Ser392) antibody.
Western blot analysis of extracts from 293 cells treated with Etoposide (25uM, 60mins) and COS7 cells treated with EGF( 200ng/ml, 30mins), using p53 (Phospho- Ser392) antibody.
Western blot analysis of extracts from 293 cells treated with Etoposide (25uM, 60mins) and COS7 cells treated with EGF( 200ng/ml, 30mins), using p53 (Phospho- Ser392) antibody.
Immunofluorescence analysis of HeLa cells, using p53 (Phospho-Ser392) antibody.
Immunofluorescence analysis of HeLa cells, using p53 (Phospho-Ser392) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0530A-Conjugate. Price: $485 / Size: 100μl.

E11-0530A-FITC$485 / 100μlE11-0530A-Biotin$485 / 100μlE11-0530A-HRP$485 / 100μlE11-0530A-AP$485 / 100μlE11-0530A-AF350$485 / 100μlE11-0530A-AF405M$485 / 100μlE11-0530A-AF488$485 / 100μlE11-0530A-AF514$485 / 100μlE11-0530A-AF532$485 / 100μlE11-0530A-AF555$485 / 100μlE11-0530A-AF568$485 / 100μlE11-0530A-AF405S$485 / 100μlE11-0530A-AF405L$485 / 100μlE11-0530A-AF546$485 / 100μlE11-0530A-AF594$485 / 100μlE11-0530A-AF610$485 / 100μlE11-0530A-AF635$485 / 100μlE11-0530A-AF647$485 / 100μlE11-0530A-AF680$485 / 100μlE11-0530A-AF700$485 / 100μlE11-0530A-AF750$485 / 100μlE11-0530A-AF790$485 / 100μlE11-0530A-APC$485 / 100μlE11-0530A-PE$485 / 100μlE11-0530A-Cy3$485 / 100μlE11-0530A-Cy5$485 / 100μlE11-0530A-Cy5.5$485 / 100μlE11-0530A-Cy7$485 / 100μlE11-0530A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.