Other Names E1A-associated protein p300, EC 2.3.1.48, EP300, P300 transcription coactivator homolog
All Sites Human: Ser89; Mouse: Ser89; Rat: Ser89
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human p300 around the phosphorylation site of serine 89 (S-G-SP-S-P).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity p300 (Phospho-Ser89) antibody detects endogenous levels of p300 only when phosphorylated at serine 89.
ReferencesEckner R., Genes Dev. 8:869-884(1994).
Dunham I., Nature 402:489-495(1999).
Chaffanet M., Genes Chromosomes Cancer 28:138-144(2000).