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Primary Antibodies

n-NOS (Phospho-Ser852) Antibody

E11-0527ACite ID: EN0017205DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000
P29476
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names BNOS, Constitutive NOS, EC 1.14.13.39, N-NOS, NC-NOS, NOS, type I, NOS1, Neuronal NOS, Nitric-oxide synthase, brain, bNOS
All Sites Human: Ser852; Mouse: Ser847; Rat: Ser847
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human n-NOS around the phosphorylation site of serine 847 (F-N-SP-V-S).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity n-NOS (Phospho-Ser852) antibody detects endogenous levels of n-NOS only when phosphorylated at serine 847.
ReferencesGeorge D. Webb, J. Thorac. Cardiovasc. Surg., Nov 2006; 132: 1131 - 1136.
Montserrat García–Vitoria, J. Histochem. Cytochem., Aug 2000; 48: 1111.
Peter J. Reiser, J Appl Physiol, Apr 1997; 82: 1250.
VL Dawson, J. Neurosci., Apr 1996; 16: 2479.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemistry analysis of paraffin-embedded human brain tissue using n-NOS (Phospho-Ser852) antibody.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using n-NOS (Phospho-Ser852) antibody.
Western blot analysis of extracts from A549 cells, using n-NOS (Phospho-Ser852) antibody.
Western blot analysis of extracts from A549 cells, using n-NOS (Phospho-Ser852) antibody.
Immunofluorescence analysis of HeLa cells, using n-NOS (Phospho-Ser852) antibody.
Immunofluorescence analysis of HeLa cells, using n-NOS (Phospho-Ser852) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0527A-Conjugate. Price: $485 / Size: 100μl.

E11-0527A-FITC$485 / 100μlE11-0527A-Biotin$485 / 100μlE11-0527A-HRP$485 / 100μlE11-0527A-AP$485 / 100μlE11-0527A-AF350$485 / 100μlE11-0527A-AF405M$485 / 100μlE11-0527A-AF488$485 / 100μlE11-0527A-AF514$485 / 100μlE11-0527A-AF532$485 / 100μlE11-0527A-AF555$485 / 100μlE11-0527A-AF568$485 / 100μlE11-0527A-AF405S$485 / 100μlE11-0527A-AF405L$485 / 100μlE11-0527A-AF546$485 / 100μlE11-0527A-AF594$485 / 100μlE11-0527A-AF610$485 / 100μlE11-0527A-AF635$485 / 100μlE11-0527A-AF647$485 / 100μlE11-0527A-AF680$485 / 100μlE11-0527A-AF700$485 / 100μlE11-0527A-AF750$485 / 100μlE11-0527A-AF790$485 / 100μlE11-0527A-APC$485 / 100μlE11-0527A-PE$485 / 100μlE11-0527A-Cy3$485 / 100μlE11-0527A-Cy5$485 / 100μlE11-0527A-Cy5.5$485 / 100μlE11-0527A-Cy7$485 / 100μlE11-0527A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.