Other Names NFKB3, Nuclear factor NF-kappa-B p65 subunit, RELA, TF65, Transcription factor p65, p65
All Sites Human: Thr505; Mouse: Thr504; Rat: Thr505
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human NF-κB p65 around the phosphorylation site of threonine 505 (L-V-TP-G-A).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity NF-κB p65 (Phospho-Thr505) antibody detects endogenous levels of NF-κB p65 only when phosphorylated at threonine 505.
ReferencesRuben S.M., Science 251:1490-1493(1991).
Deloukas P., Hum. Mol. Genet. 2:1895-1900(1993).
Lyle R., Gene 138:265-266(1994).