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Primary Antibodies

MYPT1 (Phospho-Thr853) Antibody

E11-0518ACite ID: EN0017193DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
O14974
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names MBS, Myosin phosphatase target subunit 1, PPP1R12A
All Sites Human: Thr853; Mouse: Thr852; Rat: Thr855
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human MYPT1 around the phosphorylation site of threonine 853 (R-S-TP-G-V).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity MYPT1 (Phospho-Thr853) antibody detects endogenous levels of MYPT1 only when phosphorylated at threonine 853.
ReferencesKevin B. Atkins, Am J Physiol Heart Circ Physiol, Jul 2007; 293: H402 - H408.
Allison M. Given, Am J Physiol Cell Physiol, Jan 2007; 292: C432 - C439.
Haiying Zhang, Circ. Res., Mar 2007; 100: 730 - 737.
Mikiharu Yoshida, J. Biochem. (Tokyo), Feb 1988; 103: 380 - 385.
Price225$
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using (Phospho-Thr853) antibody.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using (Phospho-Thr853) antibody.
Western blot analysis of extracts from MYPT1 NIH/3T3 cells, using MYPT1 (Phospho-Thr853) antibody.
Western blot analysis of extracts from MYPT1 NIH/3T3 cells, using MYPT1 (Phospho-Thr853) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0518A-Conjugate. Price: $485 / Size: 100μl.

E11-0518A-FITC$485 / 100μlE11-0518A-Biotin$485 / 100μlE11-0518A-HRP$485 / 100μlE11-0518A-AP$485 / 100μlE11-0518A-AF350$485 / 100μlE11-0518A-AF405M$485 / 100μlE11-0518A-AF488$485 / 100μlE11-0518A-AF514$485 / 100μlE11-0518A-AF532$485 / 100μlE11-0518A-AF555$485 / 100μlE11-0518A-AF568$485 / 100μlE11-0518A-AF405S$485 / 100μlE11-0518A-AF405L$485 / 100μlE11-0518A-AF546$485 / 100μlE11-0518A-AF594$485 / 100μlE11-0518A-AF610$485 / 100μlE11-0518A-AF635$485 / 100μlE11-0518A-AF647$485 / 100μlE11-0518A-AF680$485 / 100μlE11-0518A-AF700$485 / 100μlE11-0518A-AF750$485 / 100μlE11-0518A-AF790$485 / 100μlE11-0518A-APC$485 / 100μlE11-0518A-PE$485 / 100μlE11-0518A-Cy3$485 / 100μlE11-0518A-Cy5$485 / 100μlE11-0518A-Cy5.5$485 / 100μlE11-0518A-Cy7$485 / 100μlE11-0518A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.