Other Names MBS, Myosin phosphatase target subunit 1, PPP1R12A
All Sites Human: Thr853; Mouse: Thr852; Rat: Thr855
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human MYPT1 around the phosphorylation site of threonine 853 (R-S-TP-G-V).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity MYPT1 (Phospho-Thr853) antibody detects endogenous levels of MYPT1 only when phosphorylated at threonine 853.
ReferencesKevin B. Atkins, Am J Physiol Heart Circ Physiol, Jul 2007; 293: H402 - H408.
Allison M. Given, Am J Physiol Cell Physiol, Jan 2007; 292: C432 - C439.
Haiying Zhang, Circ. Res., Mar 2007; 100: 730 - 737.
Mikiharu Yoshida, J. Biochem. (Tokyo), Feb 1988; 103: 380 - 385.