Other Names C-myb; Myb proto-oncogene protein
All Sites Human: Ser12; Mouse: Ser12
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human MYB around the phosphorylation site of serine 12 (Y-S-SP-D-E).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity MYB (Phospho-Ser12) antibody detects endogenous levels of MYB only when phosphorylated at serine 12.
ReferencesMajello B., Proc. Natl. Acad. Sci. U.S.A. 83:9636-9640(1986).
Westin E.H., Oncogene 5:1117-1124(1990).
Dasgupta P., Oncogene 4:1419-1423(1989).