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Primary Antibodies

IRS-1 (Phospho-Ser323) Antibody

E11-0497ACite ID: EN0017158DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000
P35568
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names IRS-1, IRS1, Insulin receptor substrate-1
All Sites Human: Ser323; Mouse: Ser318; Rat: Ser318
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 323 (P-G-SP-F-R).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity IRS-1 (Phospho-Ser323) antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 323.
ReferencesJoëlle Dupont, Am J Physiol Endocrinol Metab, Feb 1998; 274: E309 - E316.
DG Baskin, Endocrinology, Apr 1994; 134: 1952.
Zhefu Ma,. Mol. Cell. Biol., Dec 2006; 26: 9338 - 9351.
Alexandros Tzatsos, J. Biol. Chem., Jun 2007; 282: 18069 - 18082.
Price225$
Western blot analysis of extracts from COS7 cells, treated with Serum (20%, 15mins), using IRS-1 (Phospho-Ser323) antibody.
Western blot analysis of extracts from COS7 cells, treated with Serum (20%, 15mins), using IRS-1 (Phospho-Ser323) antibody.
📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E11-0497A-Conjugate. Price: $485 / Size: 100μl.

E11-0497A-FITC$485 / 100μlE11-0497A-Biotin$485 / 100μlE11-0497A-HRP$485 / 100μlE11-0497A-AP$485 / 100μlE11-0497A-AF350$485 / 100μlE11-0497A-AF405M$485 / 100μlE11-0497A-AF488$485 / 100μlE11-0497A-AF514$485 / 100μlE11-0497A-AF532$485 / 100μlE11-0497A-AF555$485 / 100μlE11-0497A-AF568$485 / 100μlE11-0497A-AF405S$485 / 100μlE11-0497A-AF405L$485 / 100μlE11-0497A-AF546$485 / 100μlE11-0497A-AF594$485 / 100μlE11-0497A-AF610$485 / 100μlE11-0497A-AF635$485 / 100μlE11-0497A-AF647$485 / 100μlE11-0497A-AF680$485 / 100μlE11-0497A-AF700$485 / 100μlE11-0497A-AF750$485 / 100μlE11-0497A-AF790$485 / 100μlE11-0497A-APC$485 / 100μlE11-0497A-PE$485 / 100μlE11-0497A-Cy3$485 / 100μlE11-0497A-Cy5$485 / 100μlE11-0497A-Cy5.5$485 / 100μlE11-0497A-Cy7$485 / 100μlE11-0497A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.

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