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Primary Antibodies

IRF-3 (Phospho-Ser385) Antibody

E11-0496ACite ID: EN0017156DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000
Q14653
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names Interferon regulatory factor 3; IRF3
All Sites Human: Ser385; Mouse: Ser387; Rat: Ser380
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human IRF-3 around the phosphorylation site of serine 385 (G-A-SP-S-L).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity IRF-3 (Phospho-Ser385) antibody detects endogenous levels of IRF-3 only when phosphorylated at serine 385.
ReferencesAu W.W.-C., Proc. Natl. Acad. Sci. U.S.A. 92:11657-11661(1995).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Bellingham J., Ann. Hum. Genet. 62:231-234(1998).
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using IRF-3 (Phospho- Ser385) antibody.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using IRF-3 (Phospho- Ser385) antibody.
Western blot analysis of extracts from HT-29 cells, treated with insulin (0.01U/ml, 15mins), using IRF-3 (Phospho-Ser385) antibody.
Western blot analysis of extracts from HT-29 cells, treated with insulin (0.01U/ml, 15mins), using IRF-3 (Phospho-Ser385) antibody.
Immunofluorescence analysis of HeLa cells, using IRF-3 (Phospho-Ser385) antibody.
Immunofluorescence analysis of HeLa cells, using IRF-3 (Phospho-Ser385) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0496A-Conjugate. Price: $485 / Size: 100μl.

E11-0496A-FITC$485 / 100μlE11-0496A-Biotin$485 / 100μlE11-0496A-HRP$485 / 100μlE11-0496A-AP$485 / 100μlE11-0496A-AF350$485 / 100μlE11-0496A-AF405M$485 / 100μlE11-0496A-AF488$485 / 100μlE11-0496A-AF514$485 / 100μlE11-0496A-AF532$485 / 100μlE11-0496A-AF555$485 / 100μlE11-0496A-AF568$485 / 100μlE11-0496A-AF405S$485 / 100μlE11-0496A-AF405L$485 / 100μlE11-0496A-AF546$485 / 100μlE11-0496A-AF594$485 / 100μlE11-0496A-AF610$485 / 100μlE11-0496A-AF635$485 / 100μlE11-0496A-AF647$485 / 100μlE11-0496A-AF680$485 / 100μlE11-0496A-AF700$485 / 100μlE11-0496A-AF750$485 / 100μlE11-0496A-AF790$485 / 100μlE11-0496A-APC$485 / 100μlE11-0496A-PE$485 / 100μlE11-0496A-Cy3$485 / 100μlE11-0496A-Cy5$485 / 100μlE11-0496A-Cy5.5$485 / 100μlE11-0496A-Cy7$485 / 100μlE11-0496A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.