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Primary Antibodies

IKK-β (Phospho-Tyr199) Antibody

E11-0490ACite ID: EN0017145DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
O14920
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names EC 2.7.11.10, I-kappa-B kinase 2, I-kappa-B-kinase beta, IKK-B, IKK-beta, IKK2, IKKB, IkBKB, NFKBIKB, Nuclear factor NF-kappa-B inhibitor kinase beta, kinase IKK-beta
All Sites Human: Tyr199; Mouse: Tyr199; Rat: Tyr199
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human IKK-β around the phosphorylation site of tyrosine 199 (Q-K-YP-T-V).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity IKK-β (Phospho-Tyr199) antibody detects endogenous levels of IKK-β only when phosphorylated at tyrosine 199.
ReferencesZhi-Wei Li, J. Immunol., May 2003; 170: 4630.
Wen-Hui Hu, J. Biol. Chem., Aug 2005; 280: 29233 - 29241.
S. Escotte, Eur. Respir. J., Apr 2003; 21: 574 - 581.
Dirk Haller, J. Biol. Chem., Oct 2002; 277: 38168 - 38178.
Price225$
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using (Phospho-Tyr199) antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using (Phospho-Tyr199) antibody.
Western blot analysis of extracts from HeLa IKK-β cells, treated with TNF-a (20ng/ml) and Calyculin (50nM, 5mins), using IKK-β (Phospho-Tyr199) antibody.
Western blot analysis of extracts from HeLa IKK-β cells, treated with TNF-a (20ng/ml) and Calyculin (50nM, 5mins), using IKK-β (Phospho-Tyr199) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0490A-Conjugate. Price: $485 / Size: 100μl.

E11-0490A-FITC$485 / 100μlE11-0490A-Biotin$485 / 100μlE11-0490A-HRP$485 / 100μlE11-0490A-AP$485 / 100μlE11-0490A-AF350$485 / 100μlE11-0490A-AF405M$485 / 100μlE11-0490A-AF488$485 / 100μlE11-0490A-AF514$485 / 100μlE11-0490A-AF532$485 / 100μlE11-0490A-AF555$485 / 100μlE11-0490A-AF568$485 / 100μlE11-0490A-AF405S$485 / 100μlE11-0490A-AF405L$485 / 100μlE11-0490A-AF546$485 / 100μlE11-0490A-AF594$485 / 100μlE11-0490A-AF610$485 / 100μlE11-0490A-AF635$485 / 100μlE11-0490A-AF647$485 / 100μlE11-0490A-AF680$485 / 100μlE11-0490A-AF700$485 / 100μlE11-0490A-AF750$485 / 100μlE11-0490A-AF790$485 / 100μlE11-0490A-APC$485 / 100μlE11-0490A-PE$485 / 100μlE11-0490A-Cy3$485 / 100μlE11-0490A-Cy5$485 / 100μlE11-0490A-Cy5.5$485 / 100μlE11-0490A-Cy7$485 / 100μlE11-0490A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.