Other Names HSPB6, Heat-shock 20 kDa like-protein p20
All Sites Human: Ser16; Mouse: Ser16; Rat: Ser16
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human HSP20 around the phosphorylation site of serine 16 (R-A-SP-A-P).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity HSP20 (Phospho-Ser16) antibody detects endogenous levels of HSP20 only when phosphorylated at serine 16.
ReferencesGuo-Chang Fan, Circ. Res., Jun 2004; 94: 1474 - 1482.
Junzo Norimine, Infect. Immun., Feb 2004; 72: 1096 - 1106.
Marco Ventura, Appl. Envir. Microbiol., Jul 2007; 73: 4695 - 4703.
Charles R. Flynn, FASEB J, May 2003; 10.1096/fj.02-1028fje.