Other Names FKHR, FOXO1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, AF6q21 protein, FKHR2, FKHRL1, FOXO3, Forkhead box O3, Forkhead box protein O3A, Forkhead in rhabdomyosarcoma-like 1, AFX, AFX1, Afxh, Forkhead box protein O4, MLLT7, Putative forkhead domain AFX1, putative fork head domain AFX1
All Sites Human: Thr24/32; Mouse: Thr24/32; Rat: Thr24/32
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human FOXO1/3/4-pan around the phosphorylation site of threonine 24/32 (S-C-TP-W-P).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity FOXO1/3/4-pan (Phospho-Thr24/32) antibody detects endogenous levels of FOXO1/3/4-pan only when phosphorylated at threonine 24/32.
ReferencesRobert J. Southgate, J. Biol. Chem., Jul 2007; 282: 21176 - 21186.
Susumu Kodama, Mol. Cell. Biol., Sep 2004; 24: 7931 - 7940.
Dragan Marinkovic, J. Clin. Invest., Aug 2007; 117: 2133 - 2144.
Marie-Liesse Asselin-Labat, Blood, Jul 2004; 104: 215 - 223.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells