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Primary Antibodies

FOXO1/3/4-pan (Phospho-Thr24/32) Antibody

E11-0483ACite ID: EN0017135DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Q12778
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names FKHR, FOXO1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, AF6q21 protein, FKHR2, FKHRL1, FOXO3, Forkhead box O3, Forkhead box protein O3A, Forkhead in rhabdomyosarcoma-like 1, AFX, AFX1, Afxh, Forkhead box protein O4, MLLT7, Putative forkhead domain AFX1, putative fork head domain AFX1
All Sites Human: Thr24/32; Mouse: Thr24/32; Rat: Thr24/32
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human FOXO1/3/4-pan around the phosphorylation site of threonine 24/32 (S-C-TP-W-P).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity FOXO1/3/4-pan (Phospho-Thr24/32) antibody detects endogenous levels of FOXO1/3/4-pan only when phosphorylated at threonine 24/32.
ReferencesRobert J. Southgate, J. Biol. Chem., Jul 2007; 282: 21176 - 21186.
Susumu Kodama, Mol. Cell. Biol., Sep 2004; 24: 7931 - 7940.
Dragan Marinkovic, J. Clin. Invest., Aug 2007; 117: 2133 - 2144.
Marie-Liesse Asselin-Labat, Blood, Jul 2004; 104: 215 - 223.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from 293 cells, treated with Serum (20%, 15mins), using FOXO1/3/4-pan (Phospho-Thr24/32) antibody.
Western blot analysis of extracts from 293 cells, treated with Serum (20%, 15mins), using FOXO1/3/4-pan (Phospho-Thr24/32) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0483A-Conjugate. Price: $485 / Size: 100μl.

E11-0483A-FITC$485 / 100μlE11-0483A-Biotin$485 / 100μlE11-0483A-HRP$485 / 100μlE11-0483A-AP$485 / 100μlE11-0483A-AF350$485 / 100μlE11-0483A-AF405M$485 / 100μlE11-0483A-AF488$485 / 100μlE11-0483A-AF514$485 / 100μlE11-0483A-AF532$485 / 100μlE11-0483A-AF555$485 / 100μlE11-0483A-AF568$485 / 100μlE11-0483A-AF405S$485 / 100μlE11-0483A-AF405L$485 / 100μlE11-0483A-AF546$485 / 100μlE11-0483A-AF594$485 / 100μlE11-0483A-AF610$485 / 100μlE11-0483A-AF635$485 / 100μlE11-0483A-AF647$485 / 100μlE11-0483A-AF680$485 / 100μlE11-0483A-AF700$485 / 100μlE11-0483A-AF750$485 / 100μlE11-0483A-AF790$485 / 100μlE11-0483A-APC$485 / 100μlE11-0483A-PE$485 / 100μlE11-0483A-Cy3$485 / 100μlE11-0483A-Cy5$485 / 100μlE11-0483A-Cy5.5$485 / 100μlE11-0483A-Cy7$485 / 100μlE11-0483A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.