Other Names EC 2.7.10.2, FADK 1, FAK1, Focal adhesion kinase 1, PTK2, Protein- tyrosine kinase 2, pp125FAK
All Sites Human: Tyr407; Mouse: Tyr438; Rat: Tyr407
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human FAK around the phosphorylation site of tyrosine 407 (D-T-YP-T-M).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity FAK (Phospho-Tyr407) antibody detects endogenous levels of FAK only when phosphorylated at tyrosine 407.
ReferencesGraham Jones. Cancer Res., Aug 2001; 61: 5688 - 5691.
Chuanhai Zheng. J. Biol. Chem., Jan 1998; 273: 2384.
Ian S. Hitchcock. Blood (ASH Annual Meeting Abstracts), Nov 2006; 108: 354.