Other Names ETS-domain transcription factor ERF, Ets2 repressor factor
All Sites Human: Thr526; Mouse: Thr529
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human ERF around the phosphorylation site of threonine 526 (P-L-TP-P-R).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity ERF (Phospho-Thr526) antibody detects endogenous levels of ERF only when phosphorylated at threonine 526.
ReferencesChara Papadaki, Mol. Cell. Biol., Jul 2007; 27: 5201 - 5213.
Lionel Le Gallic, Mol. Cell. Biol., Feb 2004; 24: 1206 - 1218.
Lisa A. McPherson, PNAS, Apr 1997; 94: 4342.
Luis Oñate-Sánchez, Plant Physiology, Jan 2007; 143: 400 - 409.