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Primary Antibodies

Estrogen Receptor-α (Phospho-Tyr537) Antibody

E11-0476ACite ID: EN0017121DatasheetCOAMSDS
Human, Mouse
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
P03372
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names ER, ESR, ESR1, ESTR, ESTRA, Estradiol receptor, Estrogen receptor, NR3A1
All Sites Human: Tyr537; Mouse: Tyr451
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human ER-α around the phosphorylation site of tyrosine 537 (P-L-YP-D-L).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity Estrogen Receptor-α (Phospho-Tyr537) antibody detects endogenous levels of ER-α only when phosphorylated at tyrosine 537.
ReferencesSehime Temel, Endocrinology, Oct 2002; 143: 3974.
Xin-Tian Zhang, AACR Meeting Abstracts, Apr 2005; 2005: 302.
Suwit J. Somponpun, Am J Physiol Regulatory Integrative Comp Physiol, Sep 2004; 287: R661 - R669.
Marcello Maggiolini, Cancer Res., Oct 1999; 59: 4864 - 4869.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Estrogen Receptor-α (Phospho-Tyr537) antibody.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Estrogen Receptor-α (Phospho-Tyr537) antibody.
Western blot analysis of extracts from 293 cells, using Estrogen Receptor-α (Phospho- Tyr537) antibody.
Western blot analysis of extracts from 293 cells, using Estrogen Receptor-α (Phospho- Tyr537) antibody.
analysis using Estrogen Receptor-α (Phospho-Tyr537) Antibody. Line 1: Blocking extracts from K562 cells.
analysis using Estrogen Receptor-α (Phospho-Tyr537) Antibody. Line 1: Blocking extracts from K562 cells.

Conjugated Antibody

This product is also available conjugated. Format: E11-0476A-Conjugate. Price: $485 / Size: 100μl.

E11-0476A-FITC$485 / 100μlE11-0476A-Biotin$485 / 100μlE11-0476A-HRP$485 / 100μlE11-0476A-AP$485 / 100μlE11-0476A-AF350$485 / 100μlE11-0476A-AF405M$485 / 100μlE11-0476A-AF488$485 / 100μlE11-0476A-AF514$485 / 100μlE11-0476A-AF532$485 / 100μlE11-0476A-AF555$485 / 100μlE11-0476A-AF568$485 / 100μlE11-0476A-AF405S$485 / 100μlE11-0476A-AF405L$485 / 100μlE11-0476A-AF546$485 / 100μlE11-0476A-AF594$485 / 100μlE11-0476A-AF610$485 / 100μlE11-0476A-AF635$485 / 100μlE11-0476A-AF647$485 / 100μlE11-0476A-AF680$485 / 100μlE11-0476A-AF700$485 / 100μlE11-0476A-AF750$485 / 100μlE11-0476A-AF790$485 / 100μlE11-0476A-APC$485 / 100μlE11-0476A-PE$485 / 100μlE11-0476A-Cy3$485 / 100μlE11-0476A-Cy5$485 / 100μlE11-0476A-Cy5.5$485 / 100μlE11-0476A-Cy7$485 / 100μlE11-0476A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.