Other Names CDC28, CDC2A, CDK1, Cell division control protein 2 homolog, Cyclin-dependent kinase 1, EC 2.7.11.22, EC 2.7.11.23, MPF, kinase Cdc2, p34 protein kinase
All Sites Human: Thr14; Mouse: Thr14; Rat: Thr14
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human CDK1/CDC2 around the phosphorylation site of threonine 14 (E-G-TP-Y-G).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity CDK1/CDC2 (Phospho-Thr14) antibody detects endogenous levels of CDK1/CDC2 only when phosphorylated at threonine 14.
ReferencesCarsten Müller-Tidow, Mol. Cell. Biol., Oct 2004; 24: 8917 - 8928.
Yukinobu Arata,J. Biol. Chem., Feb 2000; 275: 6337.
Patrick Corsino, Cancer Res., Apr 2007; 67: 3135 - 3144.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells