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Primary Antibodies

CD3 ζ (Phospho-Tyr142) Antibody

E11-0468ACite ID: EN0017113DatasheetCOAMSDS
Human, Mouse
WB: 1:500~1:1000 ELISA: 1:1000
P20963
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names CD3-zeta, T-cell receptor T3 zeta chain, T-cell surface glycoprotein CD3 zeta chain precursor, T3Z, TCRZ
All Sites Human: Tyr142; Mouse: Tyr143
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human CD3 ζ around the phosphorylation site of tyrosine 142 (G-L-YP-Q-G).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity CD3 ζ (Phospho-Tyr142) antibody detects endogenous levels of CD3 ζ only when phosphorylated at tyrosine 142.
ReferencesLinda A. Trimble, Blood, Aug 2000; 96: 1021 - 1029.
Sandeep Krishnan, J. Immunol., Sep 2005; 175: 3417 - 3423.
Linda A. Trimble, J. Virol., Aug 2000; 74: 7320 - 7330.
Hisashi Arase, J. Immunol., Jan 2001; 166: 21.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from Jurkat cells, treated with UV (15mins), using CD3 ζ (Phospho-Tyr142) antibody. The
Western blot analysis of extracts from Jurkat cells, treated with UV (15mins), using CD3 ζ (Phospho-Tyr142) antibody. The
Western blot analysis using CD3 ζ (Phospho-Tyr142) Antibody. Line 1: Blocking peptide; Line 2: The extracts from JK cells ; Line 3: extracts from K562 cells.
Western blot analysis using CD3 ζ (Phospho-Tyr142) Antibody. Line 1: Blocking peptide; Line 2: The extracts from JK cells ; Line 3: extracts from K562 cells.

Conjugated Antibody

This product is also available conjugated. Format: E11-0468A-Conjugate. Price: $485 / Size: 100μl.

E11-0468A-FITC$485 / 100μlE11-0468A-Biotin$485 / 100μlE11-0468A-HRP$485 / 100μlE11-0468A-AP$485 / 100μlE11-0468A-AF350$485 / 100μlE11-0468A-AF405M$485 / 100μlE11-0468A-AF488$485 / 100μlE11-0468A-AF514$485 / 100μlE11-0468A-AF532$485 / 100μlE11-0468A-AF555$485 / 100μlE11-0468A-AF568$485 / 100μlE11-0468A-AF405S$485 / 100μlE11-0468A-AF405L$485 / 100μlE11-0468A-AF546$485 / 100μlE11-0468A-AF594$485 / 100μlE11-0468A-AF610$485 / 100μlE11-0468A-AF635$485 / 100μlE11-0468A-AF647$485 / 100μlE11-0468A-AF680$485 / 100μlE11-0468A-AF700$485 / 100μlE11-0468A-AF750$485 / 100μlE11-0468A-AF790$485 / 100μlE11-0468A-APC$485 / 100μlE11-0468A-PE$485 / 100μlE11-0468A-Cy3$485 / 100μlE11-0468A-Cy5$485 / 100μlE11-0468A-Cy5.5$485 / 100μlE11-0468A-Cy7$485 / 100μlE11-0468A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.