Other Names CBL E3 ubiquitin protein ligase, CBL2, EC 6.3.2.-, Proto-oncogene c-CBL, Signal transduction protein CBL
All Sites Human: Tyr700; Mouse: Tyr698; Rat: Tyr806
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human CBL around the phosphorylation site of tyrosine 700 (T-E-YP-M-T).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity CBL (Phospho-Tyr700) antibody detects endogenous levels of CBL only when phosphorylated at tyrosine 700.
ReferencesJoazeiro C.A., Science 286:309-312(1999).
Brdicka T., J. Exp. Med. 196:1617-1626(2002).
Salomon A.R., Proc. Natl. Acad. Sci. U.S.A. 100:443-448(2003).