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Primary Antibodies

CBL (Phospho-Tyr774) Antibody

E11-0464ACite ID: EN0017107DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000
P22681
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names CBL E3 ubiquitin protein ligase, CBL2, EC 6.3.2.-, Proto-oncogene c-CBL, Signal transduction protein CBL
All Sites Human: Tyr774; Mouse: Tyr780; Rat: Tyr888
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human CBL around the phosphorylation site of tyrosine 774 (D-G-YP-D-V).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity CBL (Phospho-Tyr774) antibody detects endogenous levels of CBL only when phosphorylated at tyrosine 774.
ReferencesStefanie Loeser, J. Exp. Med., Apr 2007; 204: 879 - 891.
A Oda, K Ozaki, Blood, Aug 1996; 88: 1330 - 1338.
Yajun Yi, J. Immunol., Dec 2000; 165: 6627.
Ke Liang, AACR Meeting Abstracts, Apr 2005; 2005: 1296.
Price225$
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using (Phospho-Tyr774) antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using (Phospho-Tyr774) antibody.
Western blot analysis of extracts from HeLa CBL cells, treated with EGF (200ng/ml, 30mins), using CBL (Phospho-Tyr774) antibody.
Western blot analysis of extracts from HeLa CBL cells, treated with EGF (200ng/ml, 30mins), using CBL (Phospho-Tyr774) antibody.
Immunofluorescence analysis of A549 cells, using CBL (Phospho-Tyr774) antibody.
Immunofluorescence analysis of A549 cells, using CBL (Phospho-Tyr774) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0464A-Conjugate. Price: $485 / Size: 100μl.

E11-0464A-FITC$485 / 100μlE11-0464A-Biotin$485 / 100μlE11-0464A-HRP$485 / 100μlE11-0464A-AP$485 / 100μlE11-0464A-AF350$485 / 100μlE11-0464A-AF405M$485 / 100μlE11-0464A-AF488$485 / 100μlE11-0464A-AF514$485 / 100μlE11-0464A-AF532$485 / 100μlE11-0464A-AF555$485 / 100μlE11-0464A-AF568$485 / 100μlE11-0464A-AF405S$485 / 100μlE11-0464A-AF405L$485 / 100μlE11-0464A-AF546$485 / 100μlE11-0464A-AF594$485 / 100μlE11-0464A-AF610$485 / 100μlE11-0464A-AF635$485 / 100μlE11-0464A-AF647$485 / 100μlE11-0464A-AF680$485 / 100μlE11-0464A-AF700$485 / 100μlE11-0464A-AF750$485 / 100μlE11-0464A-AF790$485 / 100μlE11-0464A-APC$485 / 100μlE11-0464A-PE$485 / 100μlE11-0464A-Cy3$485 / 100μlE11-0464A-Cy5$485 / 100μlE11-0464A-Cy5.5$485 / 100μlE11-0464A-Cy7$485 / 100μlE11-0464A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.