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Primary Antibodies

Calnexin (Phospho-Ser583) Antibody

E11-0463ACite ID: EN0017105DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000
P27824
$225/ 100μg
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Other Names CALX, CANX, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90
All Sites Human: Ser583; Mouse: Ser582; Rat: Ser582
Storage/Stability Store at -20℃/1 year

Form Or Antibody

Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Calnexin around the phosphorylation site of serine 583 (N-R-SP-P-R).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/Sensitivity Calnexin (Phospho-Ser583) antibody detects endogenous levels of Calnexin only when phosphorylated at serine 583.
ReferencesShaun P Brothers, J. Mol. Endocrinol., Dec 2006; 37: 479 - 488.
Vilasack Thammavongsa, J. Biol. Chem., Sep 2005; 280: 33497 - 33505.
Yasushi Okazaki, J. Biol. Chem., Nov 2000; 275: 35751.
Milka Popov, J. Biol. Chem., Jun 1999; 274: 17635.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using (Phospho-Ser583) antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using (Phospho-Ser583) antibody.
Western blot analysis of extracts from HeLa Calnexin cells, treated with EGF (200ng/ml, 30mins), using Calnexin (Phospho-Ser583) antibody.
Western blot analysis of extracts from HeLa Calnexin cells, treated with EGF (200ng/ml, 30mins), using Calnexin (Phospho-Ser583) antibody.
Immunofluorescence analysis of HeLa cells, using Calnexin (Phospho-Ser583) antibody.
Immunofluorescence analysis of HeLa cells, using Calnexin (Phospho-Ser583) antibody.

Conjugated Antibody

This product is also available conjugated. Format: E11-0463A-Conjugate. Price: $485 / Size: 100μl.

E11-0463A-FITC$485 / 100μlE11-0463A-Biotin$485 / 100μlE11-0463A-HRP$485 / 100μlE11-0463A-AP$485 / 100μlE11-0463A-AF350$485 / 100μlE11-0463A-AF405M$485 / 100μlE11-0463A-AF488$485 / 100μlE11-0463A-AF514$485 / 100μlE11-0463A-AF532$485 / 100μlE11-0463A-AF555$485 / 100μlE11-0463A-AF568$485 / 100μlE11-0463A-AF405S$485 / 100μlE11-0463A-AF405L$485 / 100μlE11-0463A-AF546$485 / 100μlE11-0463A-AF594$485 / 100μlE11-0463A-AF610$485 / 100μlE11-0463A-AF635$485 / 100μlE11-0463A-AF647$485 / 100μlE11-0463A-AF680$485 / 100μlE11-0463A-AF700$485 / 100μlE11-0463A-AF750$485 / 100μlE11-0463A-AF790$485 / 100μlE11-0463A-APC$485 / 100μlE11-0463A-PE$485 / 100μlE11-0463A-Cy3$485 / 100μlE11-0463A-Cy5$485 / 100μlE11-0463A-Cy5.5$485 / 100μlE11-0463A-Cy7$485 / 100μlE11-0463A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.