Other Names Apoptosis regulator Bcl-2, BCL2
All Sites Human: Ser87; Mouse: Ser84; Rat: Ser84
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human BCL-2 around the phosphorylation site of serine 87 (A-L-SP-P-V).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity BCL-2 (Phospho-Ser87) antibody detects endogenous levels of BCL-2 only when phosphorylated at serine 87.
ReferencesTsujimoto Y., Proc. Natl. Acad. Sci. U.S.A. 83:5214-5218(1986).
Eguchi Y., Nucleic Acids Res. 20:4187-4192(1992).
Cleary M.L., Cell 47:19-28(1986).