Other Names Akt substrate of 160 kDa, KIAA0603, TBC1 domain family member 4, TBC4, TBCD4
All Sites Human: Thr642; Mouse: Thr649
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human AS160 around the phosphorylation site of threonine 642 (A-H-TP-F-S).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity AS160 (Phospho-Thr642) antibody detects endogenous levels of AS160 only when phosphorylated at threonine 642.
ReferencesEric B. Taylor, FASEB J, Apr 2007; 21: A1207.
Henning F. Kramer, J. Biol. Chem., Oct 2006; 281: 31478 - 31485.
Grantley R. Peck, Mol. Endocrinol., Oct 2006; 20: 2576 - 2583.