Other Names Akt1 substrate 1 (proline-rich), Lobe, MGC2865, PRAS40, proline-rich Akt substrate 40 kDa
All Sites Human: Thr246; Mouse: Thr247; Rat: Thr262
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human AKT1S1 around the phosphorylation site of threonine 246 (L-N-TP-S-D).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity Akt1 S1 (Phospho-Thr246) antibody detects endogenous levels of AKT1S1 only when phosphorylated at threonine 246.
ReferencesMayumi Yoshioka, J. Mol. Endocrinol., Aug 2007; 39: 119 - 133.
Bruno D. Fonseca, J. Biol. Chem., Aug 2007; 282: 24514 - 24524.
Kerstin Stemmer, Cancer Res., May 2007; 67: 4052 - 4068.
Linglin Xie, J. Biol. Chem., Jul 2007; 10.1074/jbc.M604857200.