Other Names ADDA, Alpha adducin, Erythrocyte adducin alpha subunit, adducin, alpha
All Sites Human: Thr445; Mouse: Thr445; Rat: Thr445
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human ADD1 around the phosphorylation site of threonine 445 (E-K-TP-R-W).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity ADD1 (Phospho-Thr445) antibody detects endogenous levels of ADD1 only when phosphorylated at threonine 445.
ReferencesJae Bum Kim, PNAS, Apr 1998; 95: 4333.
Kang Ho Kim, J. Biol. Chem., Dec 2004; 279: 51999 - 52006.
Marc Foretz, Mol. Cell. Biol., May 1999; 19: 3760 - 3768
David E. Tabor, J. Biol. Chem., Jul 1999; 274: 20603.