Other Names EC 3.1.1.79, Hormone sensitive lipase, LIPE, LIPS
All Sites Human: Ser552; Mouse: Ser557; Rat: Ser563
Storage/Stability Store at -20℃/1 year
Form Or Antibody
Form of Antibody Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human HSL around the phosphorylation site of serine 552 (R-R-SP-V-S).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/Sensitivity HSL (Phospho-Ser552) antibody detects endogenous levels of HSL only when phosphorylated at serine 552.
ReferencesMatthew J. Watt, Am J Physiol Endocrinol Metab, Mar 2006; 290: E500 - E508.
Kenji Harada, Am J Physiol Endocrinol Metab, Dec 2003; 285: E1182 - E1195.
Shu Pei Wang, Endocrinology, Dec 2004; 145: 5688 - 5693.
Mélanie Fortier, J. Lipid Res., Sep 2005; 46: 1860 - 1867.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells