ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human HDAC5 around the phosphorylation site of serine 259 (T-A-SP-E-P).
All SitesHuman: Ser259; Mouse: Ser250
Form Or Antibody
Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificty Sensitivity
Specificity/SensitivityHDAC5 (Phospho-Ser259) antibody detects endogenous levels of HDAC5 only when phosphorylated at serine 259.
ReferencesClaudie Lemercier, J. Biol. Chem., May 2000; 275: 15594.
changhoon ha, FASEB J, Apr 2007; 21: A528.
Jinjiang Pang, FASEB J, Apr 2007; 21: A1151.
Carmen C. Sucharov, Am J Physiol Cell Physiol, Nov 2006; 291: C1029 - C1037.
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells