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Primary Antibodies

Histone H3 (Phospho-Ser28) Antibody

E11-0435ACite ID: EN0017056DatasheetCOAMSDS
Human, Mouse, Rat
WB: 1:500~1:1000 ELISA: 1:1000
P68431
$225/ 100μg/100μl
Quantity
Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

All Names

AlternameH3/a, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l, HIST1H3A, histone H3.1
All SitesHuman: Ser28; Mouse: Ser28; Rat: Ser29

Form Or Antibody

Form of AntibodyRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/StabilityStore at -20℃/1 year
ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human Histone H3 around the phosphorylation site of serine 28 (R-K-SP-A-P).
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Specificty Sensitivity

Specificity/SensitivityHistone H3 (Phospho-Ser28) antibody detects endogenous levels of Histone H3 only when phosphorylated at serine 28.
ReferencesN Chodchoy, Mol. Cell. Biol., Mar 1987; 7: 1039 - 1047.
Tomoko HAYASHI, J. Biochem. (Tokyo), Jun 1984; 95: 1741 - 1749.
Scott C. Galasinski, J. Biol. Chem., Jan 2002; 277: 2579 - 2588.
Sandra B. Hake, J. Biol. Chem., Jan 2006; 281: 559 - 568.
Price225$
Research AreaAutophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
Western blot analysis of extracts from HuvEc cells, treated with Serum (20%, 30mins), using Histone H3 (Phospho- Ser28) antibody
Western blot analysis of extracts from HuvEc cells, treated with Serum (20%, 30mins), using Histone H3 (Phospho- Ser28) antibody

Conjugated Antibody

This product is also available conjugated. Format: E11-0435A-Conjugate. Price: $485 / Size: 100μl.

E11-0435A-FITC$485 / 100μlE11-0435A-Biotin$485 / 100μlE11-0435A-HRP$485 / 100μlE11-0435A-AP$485 / 100μlE11-0435A-AF350$485 / 100μlE11-0435A-AF405M$485 / 100μlE11-0435A-AF488$485 / 100μlE11-0435A-AF514$485 / 100μlE11-0435A-AF532$485 / 100μlE11-0435A-AF555$485 / 100μlE11-0435A-AF568$485 / 100μlE11-0435A-AF405S$485 / 100μlE11-0435A-AF405L$485 / 100μlE11-0435A-AF546$485 / 100μlE11-0435A-AF594$485 / 100μlE11-0435A-AF610$485 / 100μlE11-0435A-AF635$485 / 100μlE11-0435A-AF647$485 / 100μlE11-0435A-AF680$485 / 100μlE11-0435A-AF700$485 / 100μlE11-0435A-AF750$485 / 100μlE11-0435A-AF790$485 / 100μlE11-0435A-APC$485 / 100μlE11-0435A-PE$485 / 100μlE11-0435A-Cy3$485 / 100μlE11-0435A-Cy5$485 / 100μlE11-0435A-Cy5.5$485 / 100μlE11-0435A-Cy7$485 / 100μlE11-0435A-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.